Isolation, identification, partial purification, optimization and characterization of alkaline protease from Neisseria flavescence

Isolation, Purification and Characterization of a Thermophilic Alkaline Protease from Bacillus subtilis BP-36

The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...

ISOLATION, IDENTIFICATION, PARTIAL PURIFICATION, OPTIMIZATION AND CHARACTERIZATION OF ALKALINE PROTEASE FROM E. coli SHRUTI SHUKLA* AND O. P. VERMA

In the present study E. coli was used for the production of alkaline protease in eight different fermentation media. This bacterium was isolated from soil sample and screened for protease production. The enzyme production from E. coli was estimated using casein as substrate and its molecular weight was determined by SDS-PAGE. The enzyme activity was also studied at different parameters like pH,...

Isolation and Identification of an Alkaline Protease Producing Bacillus from Soil

isolation, purification and characterization of a thermophilic alkaline protease from bacillus subtilis bp-36

the goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native iranian microorganisms. at the end of screening program, a bacillus subtilis bp-36 strain producing thermophilic alkaline protease was isolated from a hot spring in ardebil province. the target enzyme was purified using a one-step aqueous two-phase systems (atps) prot...

Partial Purification and Characterization of Alkaline Protease from Bacillus sp. Isolated from Soil

Bacillus isolates were analyzed for alkaline protease production on casein containing agar plates and identified by clear zones of casein hydrolysis around colonies. Two gram positive Bacillus isolates S5 and C3 which showed the best enzyme production were studied for purification by precipitation and dialysis. The intracellular alkaline protease enzyme was purified about 4 fold with a yield of...